205 - Molecular diagnosis of sepsis: the experience atthe Pordenone hospital
Autor(s): P. Diamante, M. Avolio, S. Zamparo, S. Grosso, N. Tosoni, P. Zigante, M.L. Modolo,P. Stano, R. De Rosa, A. Camporese
Issue: RIMeL - IJLaM, Vol. 6, N. 3, 2010 (MAF Servizi srl ed.)
Page(s): 205-10
Background. It is well known that during sepsis the precocity of therapy is decisive for the prognosis and that the speed of action is associated with a specific reduction of mortality. Although blood cultures investigations is supported by the isolation of the microbiological agent, the diagnosis of sepsis remains a clinical diagnosis, as shown by literature data which show that about 15% of patients considered clinically positive for sepsis, the results blood cultures are negative. It is therefore important that a laboratory is adequately equipped to respond in the shortest possible time with a specific clinical question about the critically ill patients, and is currently investigating new molecular diagnostic strategies appear to be very promising in this respect. With this background we aimed to compare the system SeptiFast with traditional diagnostic blood cultures studying a cohort of patients who came from community to Emergency room of our hospital and selected as clinically possible patients with sepsis. Methods. 237 samples were examined: 155 (65%) gave a negative result by both methods, 82 (35%) were positive with at least one of two methods used.
Key-words: SeptiFast, sepsi molecolare, emocoltura, TAT.