275 - The dosage of homocysteine: Comparison between high-pressure liquid chromatography (HPLC) and enzymatic method (Minias) on a Olympus instrument
Autor(s): A. Colatutto, R. Zaglia, S. Mazzolini, P. Sala
Issue: RIMeL - IJLaM, Vol. 3, N. 4, 2007 (MAF Servizi srl ed.)
Page(s): 275-278
Background. The homocysteine (HCY) is a sulphur containing amino-acid derived from the methionine metabolism. Several essays have proved the close relation between hyperhomocysteinemia and the growth of metabolic and neurodegenerative illness. Recent sperimental data have proved that the damaging effects of hyperhomocysteinemia are associated to the growth of reactive oxygen species (ROS). Polycentric studies aiming to value the diagnostic impact of the homocysteine determination as a risks factor of cardiovascular disease (CVD) have been initiated all over the world. Methods. It is necessary therefore to introduce automated methods, utilizable by small laboratories too, instead of those in HPLC and in gas chromatography, absolutely inapt to screening tests, which need compelling economic and professional resources. Our work’s purpose has been to compare the reference method HPLC-System Gold with the enzymatic method homocysteine T(HCY) of Minias on analyser Olympus AU2700. Result. The sensitivity of enzymatic resulted to be much better than the reference method. The data of the two methods come out to be completely superimposed. Elaborating the date by linear regression we noted a very good correlation between the methods under 30 mmol/L (r = 0,984). Conclusions. We believe that the enzymatic test, because of its peculiarity of trusting, repetition, easy and immediate execution and total automation can replace the procedure in HPLC.