216 - Analysis of a project designed to improve preanalytical and analytical work-flow of urine cultures.

Background. Quantitative urine culture is one of the most frequently requested tests in microbiology laboratories (about 22% in our experience). An automated system for screening purposes is strongly needed to save technical staff time and obtain rapid results. Methods. In our study we have investigated 546 urine samples collected from inpatients and outpatients with a commercial tube prepared with boric acid (Uriset, Diesse, Milano, Italy) and have compared a secondgeneration flow citometry (Sysmex UF100, Dasit, Milano, Italy) with standard urine culture tested on agar plated by means of 10 microliter loop. The specimens were screened and cultured upon receipt. Results. In our experience, the results obtained with Sysmex UF100 are very interesting, especially if this analyzer is used as screening method for negative urine samples, and comparable to data obtained from culture examination. In fact, considering together bacteria and leucocytes count (10.000 bacteria and/or 50 leucocytes/ ìL) we have obtained a negative predictive value of 0,96 in comparison to standard culture method. Conclusions. The classical culture method needs of 24 hours for a results, instead the Sysmex UF100 analyzer gives results in few minutes, thus reducing the microbiology turn around time (TAT) with obvious benefits for patients and physicians.