111 - First comparative evaluation of leucocyte differential and reticulocyte count between Abbott Cell -DYN 3700 and Beckman-Coulter Gen S
Rivista: Riv Med Lab - JLM, Vol. 4, N. 2, 2003 (SIRSE Srl ed.)
H. Vandeputte, D. Darre, F. Leroy, J. Nemmiche, C. Pucalowski, MC. Chretien.
Abstract. Background: The Cell-Dyn 3700 has
been launched with the same CBC and flagging performance
specifications as the Cell-Dyn 3500. The
primary goal of our evaluation was to evaluate the
new algorithms of its reticulocyte method and the
performance capabilities in comparison to our routine
analyser (Coulter Gen S).
Methods: The WBC differential and reticulocyte
counts were evaluated on the new Cell-Dyn 3700 instrument
(Abbott Laboratories) on 758 normal and
727 pathological samples.
Results: When compared to the routine laboratory
analyser (Gen S, Beckman Coulter) in the normal
population, similar WBC differential results were
found. In contrast, we noted some differences
within the pathological samples. A bias of 0,643
Giga/L WBCs (white blood cells) was found between
Cell-Dyn 3700 and Gen S measurements in
samples with WBC counts of less than 3 Giga/L
(no. 89). For WBC counts less than 1 Giga/L the
Cell-Dyn 3700 residual plots of linear regression
analysis provided the closer fit to its regression
line. FWBC (Fragile White Blood Cells) flag on
the Cell-Dyn 3700 is a good indicator of the osmotic
fragility of lymphocytes from CLL (chronic
lymphocyte leukaemia). This highlights the limited
linearity of impedance methods in these situations
at a level of 100 Giga/L and above. The Cell-
Dyn 3700 KWOC (Corrected White Optical
Count) reported value is higher than the impedance
and microscopy counts.
This study also evaluated the new Cell-Dyn 3700
reticulocyte algorithm method. In the evaluation of
400 random samples, the Cell - Dyn 3700 analyser
showed a significant bias compared to Gen S (0,4
%) in a population ranging from 0,37 to 8 % for Gen
S and 0,17 to 9,44 % for Cell-Dyn 3700 (with the
exception of one value at 14,7 %).
Conclusions: These results suggest a clear improvement
of the reticulocyte methodology on the Cell-
Dyn 3700.