033 - La Carbohydrate-Deficient Transferrin (CDT), nuovo marker di abuso alcolico cronico e la sua determinazione mediante elettroforesi capillare zonale

G. De Paoli, M. Trettene, J.P. Pascali, M. Trevisan, F. Bortolotti, F. Tagliaro Introduction: The aim of the present work was to present a brief review of the analytical methods for CDT determination and to carry out a comparison between the results obtained with a commercial immunoassay and those obtained with a capillary electrophoretic method previously validated by our group. Methods: Serum samples from 120 subjects with different alcohol consumption have been analyzed with the immunoassay %CDT-TIA (Axis Shield) in parallel with capillary electrophoresis. The immunoassay analysis has been carried out following the producer’s instructions; the capillary electrophoretic analysis has been performed using a previously validated method, based on zonal separation in borate buffer pH 8,3 with UV detection at 200 nm. Results and conclusions: Overall a highly significant (p