033 - La Carbohydrate-Deficient Transferrin (CDT), nuovo marker di abuso alcolico cronico e la sua determinazione mediante elettroforesi capillare zonale
Rivista: Riv Med Lab - JLM, Vol. 5, N. 1, 2004 (SIRSE Srl ed.)
G. De Paoli, M. Trettene, J.P. Pascali,
M. Trevisan, F. Bortolotti, F. Tagliaro
Introduction: The aim of the present work was to present a brief
review of the analytical methods for CDT determination
and to carry out a comparison between the results
obtained with a commercial immunoassay and
those obtained with a capillary electrophoretic
method previously validated by our group.
Methods: Serum samples from 120 subjects with different
alcohol consumption have been analyzed with
the immunoassay %CDT-TIA (Axis Shield) in parallel
with capillary electrophoresis. The immunoassay
analysis has been carried out following the producer’s
instructions; the capillary electrophoretic analysis
has been performed using a previously validated
method, based on zonal separation in borate buffer
pH 8,3 with UV detection at 200 nm.
Results and conclusions: Overall a highly significant
(p