283 - Misura della proteina S e della resistenza alla proteina C attivata. Risultati della Valutazione Esterna di Qualità (VEQ)-CISMEL
Rivista: Riv Med Lab - JLM, Vol. 5, N. 4, 2004 (SIRSE Srl ed.)
A. Tripodi, V. Chantarangkul
*Comitato Italiano per la Standardizzazione dei Metodi in Ematologia e Laboratorio.
Protein S and activated protein C resistance measurements.
Results of the External Quality Assurance
Scheme (EQAS) organized by the CISMEL
Background. One of the aims of the External Quality
Assurance Scheme (EQAS) is to provide information
useful to standardize laboratory methods and to
help choosing the most appropriate laboratory strategy
to investigate clinical conditions.
Methods. We looked at the results coming from the
EQAS in coagulation organized by the CISMEL
group in three different exercises where participants
analyzed three well characterized plasmas for protein
S and activated protein C resistance.
Results. Three consecutive exercises showed that the
functional anticoagulant assay for protein S is affected
by the presence of the factor V Leiden mutation
and that the method for the measurement of activated
protein C resistance based on prediluiton of
test plasma in factor V deficient plasma is highly
specific for factor V Leiden.
Conclusions. EQAS exercises carried out across the
country with samples unknown to the participants
confirm two important findings that may be used to
design a diagnostic strategy for the investigation of
patients with thrombophilia.