192 - Effetti di una nuova membrana dialitica sui recettori piastrinici GPIb e GPIIb/IIIa
Rivista: RIMeL - IJLaM, Vol. 1, N. 3, 2005 (MAF Servizi srl ed.)
M. Golato, G. Ciancaglini, F. Di Luca, F. Indino, I. Foschini, M. Liani, E. Trabassi
Effect of a new membrane for haemodialysis on
platelet receptors GPIb and GPIIb/IIIa.
Background: In patients with chronic renal insufficiency, the
therapy with haemodialysis effected through the
extracorporeal circuit can induce phenomena of abnormal
biocompatibility, whose level is routinely measured through
aggregation of the platelets; the aggregation self reduces
as the illness progresses. Haemodialysis may improve the
clinical condition, but it is not possible to renew the
physiological aggregation and adhesion of the platelets.
Therefore, patients with chronic renal insufficiency develop
a condition of pro-thrombosis, whose pathogenesis is
caused by an abnormal expression of the superficial platelet
receptors (SPR) GPIb and GPIIb/IIIa. The objective of
this study was to evaluate the effects of a new polysulfonate
(PSN) membrane for dialysis on the SPR.
Methods: The monoclonal antibodies CD41a and CD41b
were used to measure the expression of SPR on platelets
after dialysis with conventional and PSN membranes. The
results were read by means of Fascalibur (BD) flow
cytometry.
Results and conclusions: the results show that different
techniques and membranes for dialysis can produce
different effects on the expression of the platelet receptors
depending on the characteristics of the bio-compatibility.
In particular, the membrane for dialysis in PSN induces a
reduction in the expression of RPS GPIIb/IIIa,
comparable with the effects of peritoneal dialysis on the
same receptors.