192 - Effetti di una nuova membrana dialitica sui recettori piastrinici GPIb e GPIIb/IIIa

M. Golato, G. Ciancaglini, F. Di Luca, F. Indino, I. Foschini, M. Liani, E. Trabassi Effect of a new membrane for haemodialysis on platelet receptors GPIb and GPIIb/IIIa. Background: In patients with chronic renal insufficiency, the therapy with haemodialysis effected through the extracorporeal circuit can induce phenomena of abnormal biocompatibility, whose level is routinely measured through aggregation of the platelets; the aggregation self reduces as the illness progresses. Haemodialysis may improve the clinical condition, but it is not possible to renew the physiological aggregation and adhesion of the platelets. Therefore, patients with chronic renal insufficiency develop a condition of pro-thrombosis, whose pathogenesis is caused by an abnormal expression of the superficial platelet receptors (SPR) GPIb and GPIIb/IIIa. The objective of this study was to evaluate the effects of a new polysulfonate (PSN) membrane for dialysis on the SPR. Methods: The monoclonal antibodies CD41a and CD41b were used to measure the expression of SPR on platelets after dialysis with conventional and PSN membranes. The results were read by means of Fascalibur (BD) flow cytometry. Results and conclusions: the results show that different techniques and membranes for dialysis can produce different effects on the expression of the platelet receptors depending on the characteristics of the bio-compatibility. In particular, the membrane for dialysis in PSN induces a reduction in the expression of RPS GPIIb/IIIa, comparable with the effects of peritoneal dialysis on the same receptors.